Journal Article

Characterization of a Calcium-Dependent Protein Kinase of Tobacco Leaves That Is Associated with the Plasma Membrane and Is Inducible by Sucrose

Yukiko Iwata, Masamitsu Kuriyama, Makiko Nakakita, Hisae Kojima, Masa-aki Ohto and Kenzo Nakamura

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 39, issue 11, pages 1176-1183
Published in print November 1998 | ISSN: 0032-0781
Published online November 1998 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029318
Characterization of a Calcium-Dependent Protein Kinase of Tobacco Leaves That Is Associated with the Plasma Membrane and Is Inducible by Sucrose

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The plasma membrane fraction from leaves of tobacco contains a 54-kDa protein with autophosphorylation activity, and the level of this protein increases after feeding of leaves with sucrose [Ohto and Nakamura (1995) Plant Physiol. 109: 973]. The 54-kDa autophosphorylation protein could not be released from the plasma membrane by treatment with salt or alkali but could be efficiently solubi-lized by 1% sodium deoxycholate (NaDOC). Ion-exchange chromatography of the NaDOC-solubilized proteins in the presence of octylglucoside separated the 54-kDa autophosphorylation protein into three peaks. The autophosphorylation activity of the 54-kDa protein in peaks I and II increased after feeding of leaves with sucrose. The 54-kDa protein in the peak II fraction was enriched about 125-fold starting from the microsomal membrane fraction. The 54-kDa protein in this fraction phosphorylated histone HIS in a calcium-dependent manner and cross-reacted with an antibody against a calcium-dependent protein kinase (CDPK) of Arabidopsis thaliana. These results suggest that the 54-kDa protein in the peak II fraction is a novel isoform of CDPK which is associated with the plasma membrane and is inducible by sucrose.

Keywords: CDPK; Plasma membrane; Protein kinase; Sugar-induced gene expression; Tobacco (Nicotiana tabacum)

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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