Journal Article

Differential Expression of Genes Involved in the Biosynthesis and Perception of Ethylene during Ripening of Passion Fruit <i>(Passiflora edulis</i> Sims)

Satoru Mita, Syoichiro Kawamura, Kazuki Yamawaki, Kenzo Nakamura and Hiroshi Hyodo

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 39, issue 11, pages 1209-1217
Published in print November 1998 | ISSN: 0032-0781
Published online November 1998 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029322
Differential Expression of Genes Involved in the Biosynthesis and Perception of Ethylene during Ripening of Passion Fruit (Passiflora edulis Sims)

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An increase in the enzyme activity of 1-aminocyclopropane-1-carboxylic acid (ACC) synthase and ACC oxidase induces the evolution of ethylene during the ripening of passion fruit. A much higher level of ethylene is produced in arils than in seeds or peels during ripening. The pattern of expression of two ACC synthase genes (PE-ACS1 and PE-ACS2), one ACC oxidase gene (PE-ACO1), and two ethylene receptor genes (PE-ETR1 and PE-ERS1) revealed that the expression of these genes is differentially regulated. Expression of PE-ACS1 and PE-ACO1 was enhanced during ripening and after ethylene treatment. However, prominent expression of PE-ACS1 was delayed compared to that of PE-ACO1. Much larger quantities of PE-ACS1 mRNA and PE-ACO1 mRNA were seen in arils than in seeds; this corresponds well with an increase in the amount of ethylene produced by the plant tissue itself. The level of PE-ACS2 mRNA was detectable in arils of the preclimacteric fruit, although it decreased during ripening. These results suggest that expression of PE-ACS1 and PE-ACO1 is required to increase the activity of ethylene biosynthetic enzymes during ripening. The level of expression of PE-ETR1 and PE-ERS1 did not significantly change over the course of ripening; however, the mRNA levels of PE-ETR1 and PE-ERS1 were much higher in arils than in seeds.

Keywords: ACC oxidase; ACC synthase; Ethylene receptor; Passion fruit; Ripening

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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