Journal Article

Physiological Mechanisms of a Sub-Systemic Oxidative Burst Triggered by Elicitor-Induced Local Oxidative Burst in Potato Tuber Slices

Hae-Jun, Park, Yoshio Miura, Kazuhito Kawakita, Hirofumi Yoshioka and Noriyuki Doke

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 39, issue 11, pages 1218-1225
Published in print November 1998 | ISSN: 0032-0781
Published online November 1998 | e-ISSN: 1471-9053 | DOI: https://dx.doi.org/10.1093/oxfordjournals.pcp.a029323
Physiological Mechanisms of a Sub-Systemic Oxidative Burst Triggered by Elicitor-Induced Local Oxidative Burst in Potato Tuber Slices

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Physiological mechanisms of triggering and occurrence of a short-distance systemic (sub-systemic) oxidative burst (OXB) caused by induction of local OXB induced by the elicitor were investigated using potato tuber tissues. The sub-systemic OXB was detected as luminol-mediated chemiluminescence (CL) on the non-treated side of tissue slices following a transient appearance of local OXB on the other side directly treated with an elicitor. The sub-systemic OXB was not induced when the elicitor was applied in the presence of a radical scavenger, H2O2-catabolising enzyme, or inhibitors of activation of local OXB and NADPH oxidase (diphenyleneiodonium: DPI), Ca2+ che-lator and Ca2+ channel blockers). Treatment with H2O2 solution rapidly caused the sub-systemic OXB, which was inhibited by the presence of the above inhibitors either during the treatment with H2O2 or detection of CL. These results suggested that the elicitor-stimulated sub-systemic OXB may be dependent on Of generating NADPH oxidase activated by an unknown systemic signal stimulated by H2O2 generated via local activation of the NADPH oxidase.

Keywords: Ca2+ signaling; Local oxidative burst; Luminol-mediated chemiluminescence; O2- generating NADPH oxidase; Sub-systemic oxidative burst; Systemic oxidative burst; Systemic signal

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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