Journal Article

Identification of Polypeptides Associated with an Enriched Cytoskeleton-Protein Body Fraction from Developing Rice Endosperm

Yujia Wu, Douglas G. Muench, Yeong-Tae Kim, Young-Soo Hwang and Thomas W. Okita

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 39, issue 12, pages 1251-1257
Published in print December 1998 | ISSN: 0032-0781
Published online December 1998 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029328
Identification of Polypeptides Associated with an Enriched Cytoskeleton-Protein Body Fraction from Developing Rice Endosperm

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  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Recent evidence has shown that the prolamine polysomes are attached not only to the endoplasmic reticulum membranes that bound the prolamine protein bodies (PBs) but also to cytoskeleton elements associated with this sub-cellular fraction. To learn more about the nature of the proteins that are associated with this supra-macromolecular complex, proteins extracted from an enriched cytoskeleton-PB fraction were resolved by two-dimensional polyacryl-amide gel electrophoresis under non-equilibrium conditions and analyzed for their composition by immunological and biochemical methods. Immunoblot analysis indicated the presence of the cytoskeletal proteins, actin and tubulin, and the cytoskeletal-associated protein EFα in this fraction. Microsequencing of selected polypeptides revealed a diversity of protein sequences. In addition to contaminating storage proteins which are selectively solubilized by the isolation procedure, several ribosomal proteins and histone H3 were also identified. Some of the remaining polypeptides showed partial homology to protein sequences deposited in the database, several of which are cytoskele-ton-associated proteins.

Keywords: Cytoskeleton; Edman degradation; Immunoblot; Oryza sativa; Protein bodies.

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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