Journal Article

Modulation by Bicarbonate of Catalytic and Regulatory Properties of C<sub>4</sub>Phospho<i>enol</i>pyruvate Carboxylase from <i>Amaranthus hypochondriacus</i>: Desensitization to Malate and Glucose 6-Phosphate and Sensitization to Mg<sup>2+</sup>

K. Parvathi, A.S. Bhagwat and A.S. Raghavendra

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 39, issue 12, pages 1294-1298
Published in print December 1998 | ISSN: 0032-0781
Published online December 1998 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029333
Modulation by Bicarbonate of Catalytic and Regulatory Properties of C4Phosphoenolpyruvate Carboxylase from Amaranthus hypochondriacus: Desensitization to Malate and Glucose 6-Phosphate and Sensitization to Mg2+

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The catalytic and regulatory properties of phosphoenolpyruvate (PEP) carboxylase (PEPC) are modulated remarkably by the increase in the level of bicarbonate in the assay medium. The activity of PEPC increased by two-fold as the concentration of bicarbonate was raised from 0.05 to 10 mM. During this state, there was only marginal effect on Km for PEP, while the affinity of PEPC to Mg2+ increased by >2 fold. In contrast, the sensitivity of PEPC to malate decreased with increasing concentration of HCO3. Similarly, the stimulation by glucose 6-phosphate (G-6-P) at optimal concentration (10 mM) of HCO3 was much less than that at suboptimal concentration (0.05 mM). K1 for malate increased by about 3 fold and Ka for G-6-P rised by fourfold as bicarbonate concentration was rised from 0.05 to 10 mM. These results suggest that HCO3 desensitizes PEPC to both malate and G-6-P. Further, these changes were manifested in both dark- as well as light-forms of the enzyme. Similar results were obtained with PEPC in leaf extracts or in purified form. We therefore propose that bicarbonate-induced changes are independent of phospho-rylation and possibly through a significant change in the conformation of the enzyme. This is the first detailed report indicating marked modulation of regulatory and catalytic properties of PEPC by bicarbonate, one of its substrate.

Keywords: Amaranthus hypochondriacus; Bicarbonate; Malate sensitivity; Phosphoenolpyruvate carboxylase; Regulatory properties

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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