Journal Article

Cloning of NAD-Dependent Sorbitol Dehydrogenase from Apple Fruit and Gene Expression

Kunio Yamada, Yasuhito Oura, Hitoshi Mori and Shohei Yamaki

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 39, issue 12, pages 1375-1379
Published in print December 1998 | ISSN: 0032-0781
Published online December 1998 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029345
Cloning of NAD-Dependent Sorbitol Dehydrogenase from Apple Fruit and Gene Expression

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Partial amino acid sequences of NAD-dependent sorbitol dehydrogenase (NAD-SDH) were used to identify a full-length cDNA from apple fruit. This clone consisted of 1,433 bp containing an open reading frame of 1,137 bp that could code for a polypeptide with 379 amino acids. To our knowledge, this is the first report about cloning of NAD-SDH cDNA from a plant source. The deduced amino acids from cDNA revealed 43.7% identity to human NAD-SDH. The activity of this enzyme to convert sorbitol to fructose with the reduction of NAD was certified by the fusion protein of this clone expressed in Escherichia coli. Northern blot analysis showed that the mRNA was expressed in matured apple fruit.

Keywords: Apple (Malus domestica); cDNA cloning; NAD-dependent sorbitol dehydrogenase (EC 1.1.1.14); Sorbitol

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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