Journal Article

Identification of a Novel Triterpenoid Saponin from <i>Pisum sativum</i> as a Specific Inhibitor of the Diguanylate Cyclase of <i>Acetobacter xylinum</i>

Patricia Ohana, Deborah P. Delmer, Russell W. Carlson, John Glushka, Parastoo Azadi, Tony Bacic and Moshe Benziman

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 39, issue 2, pages 144-152
Published in print February 1998 | ISSN: 0032-0781
Published online February 1998 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029351
Identification of a Novel Triterpenoid Saponin from Pisum sativum as a Specific Inhibitor of the Diguanylate Cyclase of Acetobacter xylinum

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A specific and highly potent inhibitor of diguanylate cyclase, the key regulatory enzyme of the cellulose synthesizing apparatus in the bacterium Acetobacter xylinum, was isolated from extracts of etiolated pea shoots (Pisum sativum). The inhibitor has been purified by a multistep procedure, and sufficient amounts of highly purified compound (3-8 mg) for spectral analysis were obtained. The structure of this compound was established as 3-O-a-L-rhamnopyranosyl-(l → 2)-rβ-D-galactopyranosyl-(l → 2)-(rβ-D-glucuronopyranosyl soyasapogenol B 22-O-a-D-gluco-pyranoside. The structure was elucidated on the basis of susceptibility to various enzymes, chemical and spectral methods, such as GC-MS, FAB-MS, and the following types of 2D-NMR: COSY, ROESY, TOCSEY, HMQC, HMBC analyses. An identical or a very similar compound with identical biological activity was also isolated from A. xylinum, strongly suggesting that at least certain aspects of cellulose synthesis in the bacteria and in higher plants may be regulated in a similar manner. The content of this saponin in etiolated plants was about 0.04 μmoI (g fresh tissue)1.

Keywords: Acetobacter xylinun; Diguanylate cyclase; Inhibitor; Pisum sativum; Triterpenoid saponin

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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