Journal Article

Characterization of a cDNA Encoding CuZn-Superoxide Dismutase from the Liverwort <i>Marchantia paleacea</i> var. <i>diptera</i>

Katsuyuki Tanaka, Susumu Takio, Isamu Yamamoto and Toshio Satoh

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 39, issue 2, pages 235-240
Published in print February 1998 | ISSN: 0032-0781
Published online February 1998 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029362
Characterization of a cDNA Encoding CuZn-Superoxide Dismutase from the Liverwort Marchantia paleacea var. diptera

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Suspension-cultured cells of the liverwort Marchantia paleacea var. diptera contain a cytosolic CuZn-superoxide dismutase (SOD) whose N-terrainal amino acid sequence is similar to those of the isozymes found in chloroplasts of higher plants [Tanaka et al. (1996) Plant Cell Physiol. 37: 523]. A cDNA (MSODCc) encoding the cytosolic CuZn-SOD was isolated from cDNA library constructed from a liverwort cell suspension culture. The deduced amino acid sequence showed a higher degree of homology with the sequences of CuZn-SODs in chloroplasts than those in the cytosol of higher plants and an unique additional peptide in the C-terminal region, but no plastid transit sequence. Northern blotting using MSODCc as a probe and immunoblot analysis with antiserum against the enzyme revealed that the steady state level of transcript was not affected by copper, but both CuZn-SOD protein and its activity increased.

Keywords: cDNA sequence; Copper; Cytosolic CuZn-SOD; Liverwort cells {Marchantia paleacea var. diptera); Superoxide dismutase (EC 1.15.1.1)

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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