Journal Article

Cloning and Expression of the Soybean <i>chlH</i> Gene Encoding a Subunit of Mg-Chelatase and Localization of the Mg<sup>2+</sup> Concentration-Dependent ChlH Protein within the Chloroplast

Masato Nakayama, Tatsuru Masuda, Tsuyosbi Bando, Hiroshi Yamagata, Hiroyuki Ohta and Ken-ichiro Takamiya

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 39, issue 3, pages 275-284
Published in print March 1998 | ISSN: 0032-0781
Published online March 1998 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029368
Cloning and Expression of the Soybean chlH Gene Encoding a Subunit of Mg-Chelatase and Localization of the Mg2+ Concentration-Dependent ChlH Protein within the Chloroplast

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From the soybean cDNA library, we isolated and analyzed the chlH gene encoding a subunit of Mg-chelatase. The subunit was a polypeptide of 1,383 amino acids with a molecular mass of 153,491 Da, which shared 90% identity with the olive gene from Antirrhinum majus. The regulation of the expression of chlH was investigated in photomixotrophic soybean suspension cells (SB-P). The expression was light-inducible, and the induction was more rapid than those of chlI and cab2. Furthermore, the levels of the transcripts and products of chlH appeared to be regulated by a circadian oscillation. The subchloroplastic localization of ChlH was investigated by immunoblot analyses with antiserum against recombinant ChlH. Depending on the concentration of Mg2+ in the lysis buffer, the localization of ChlH protein migrated between the stroma and the envelope membrane; ChlH was localized on the envelope membrane, a major site of chlorophyll biosynthesis, when the Mg2+ concentration of the lysis buffer was high (above 5 mM). These results indicated that the activity of Mg-chelatase was regulated by modulation of the expression and subchloroplastic localization of ChlH protein.

Keywords: chlH; Chlorophyll biosynthesis; Circadian control; Light regulation; Protoporphyrin IX; Soybean(Glycine max)

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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