Journal Article

cDNA Cloning, Gene Expression and Secretion of Chitinase in Winged Bean

Muneharu Esaka and Takanori Teramoto

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 39, issue 3, pages 349-356
Published in print March 1998 | ISSN: 0032-0781
Published online March 1998 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029376
cDNA Cloning, Gene Expression and Secretion of Chitinase in Winged Bean

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cDNA for an acidic class III chitinase (ChitW1) was isolated from winged bean cells. The chitinase was abundantly secreted at later stages of cell culture, when levels of ChitW1 mRNA were also high. The gene was strongly expressed in roots, but a class I chitinase was strongly expressed in leaves.

Keywords: cDNA cloning; Chitinase (EC 3.2.1.14); Salicylic acid; Secretion; Senescence; Winged bean (Psophocarpus tetragonolobus)

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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