Journal Article

Immunocytochemistry of Rhamnogalacturonan II in Cell Walls of Higher Plants

Toru Matoh, Miki Takasaki, Keiji Takabe and Masaru Kobayashi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 39, issue 5, pages 483-491
Published in print May 1998 | ISSN: 0032-0781
Published online May 1998 | e-ISSN: 1471-9053 | DOI: https://dx.doi.org/10.1093/oxfordjournals.pcp.a029395
Immunocytochemistry of Rhamnogalacturonan II in Cell Walls of Higher Plants

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A polyclonal antibody against a borate-RG-II complex is raised in rabbits. The antibody recognized RG-II exclusively in cell wall polysaccharides. Immunocytochemical studies demonstrated that the epitope is ubiquitous in cell walls of all the cells in radish and rice roots, cultured tobacco cells, red clover root nodules, and lily growing pollen tubes. The label was denser in proximal to plasma membrane, and not detected in middle lamella, suggesting that borate may cross-link newly secreted pectic polysaccharides at the membrane-cell wall interface.

Keywords: AntibodyBorate-rhamnogalacturonan II complex; Cell wall; Oryza; Pollen tube; Rhaphanus

Journal Article.  0 words. 

Subjects: Plant Sciences and Forestry ; Biochemistry ; Molecular and Cell Biology

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