Journal Article

<i>RMA1</i> an <i>Arabidopsis thaliana</i> Gene Whose cDNA Suppresses the Yeast <i>secl5</i> Mutation, Encodes a Novel Protein with a RING Finger Motif and a Membrane Anchor

Noriyuki Matsuda and Akihiko Nakano

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 39, issue 5, pages 545-554
Published in print May 1998 | ISSN: 0032-0781
Published online May 1998 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029403
RMA1 an Arabidopsis thaliana Gene Whose cDNA Suppresses the Yeast secl5 Mutation, Encodes a Novel Protein with a RING Finger Motif and a Membrane Anchor

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To identify molecules that function in the plant secretory pathway, we screened for Arabidopsis thaliana cDNA clones that complement the temperature-sensitive (ts), secretion-deficient sec15 mutation of yeast Saccha-romyces cerevisiae. RMA1, one of the genes obtained in this screening, suppressed not only the ts growth of sec15 but also its secretory defect. RMA1 is not a structural homologue of SEC15 but encodes a novel 28 kDa protein with a RING finger motif and a C-terminal membrane-anchoring domain. Mutational analysis indicates that the RING finger motif of RMA1 is important for its suppression activity. In Arabidopsis plant, RMA1 is ubiquitously expressed. A search for homologous proteins in the database revealed that Arabidopsis, nematode, mouse and human possess close homologues of RMA1.

Keywords: Arabidopsis thaliana; Membrane protein; Protein secretion; Saccharomyces cerevisiae

Journal Article.  0 words. 

Subjects: Plant Sciences and Forestry ; Biochemistry ; Molecular and Cell Biology

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