Journal Article

Effects of Fluorogibberellins on Plant Growth and Gibberellin 3rβ-Hydroxylases

Tamio Saito, Yuji Kamiya, Hisakazu Yamane, Noboru Murofushi, Akira Sakurai and Nobutaka Takahashi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 39, issue 6, pages 574-580
Published in print June 1998 | ISSN: 0032-0781
Published online June 1998 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029407
Effects of Fluorogibberellins on Plant Growth and Gibberellin 3rβ-Hydroxylases

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

3rβ-Fluorogibberellin A9 (3rβ-fluoro-GA9), 3rβfluoro-GA20, 3rβ-fluorodeoxygibberellin C (3rβ-fluoro-DGC) and 13-fluoro-GA9 were prepared, and their effects on plant growth and gibberellin (GA) 3rβ-hydroxyIases were examined. 3rβ-Fluoro-GA9 and 3rβ-fluoro-GA20 promoted the growth of dwarf rice (Oryza sativa L. cv. Tan-ginbozu) seedlings to three times higher than the control seedlings at a dosage of 3 μ plant−1, and 3rβfluoro-DGC to twice higher at the same dosage. 3rβg-Fluoro-GA9 was active in cucumber (Cucumis sativus L.) hypocotyl assay, its activity being about one-thirtieth as much as that of GA4. 3rβ-Fluoro-GAs were active per se in promoting the shoot elongation of rice. 3rβ-Fluoro-DGC inhibited the 3rβ-hydroxylation of [3H2]GA9 to [3H]GA4 by GA rβ-hydroxylase from bean (Phaseolus vulgaris L.), but 3rβ-fluoro-GA9 and 3rβ-fluoro-GA20 did not show any effects on the enzyme activity. These 3rβ-fluoro-GAs also showed no or only a weak inhibitory effect on the rβ-hydroxylase from pumpkin (Cucurbita maxima L.). 13-Fluoro-GA9 promoted growth of rice and cucumber seedlings, and inhibited the 3rβ-hydroxylases from both bean and cucumber. 13-Fluoro-GA9was converted into 13-fluoro-GA4 and 2,3-didehydro-13-fluoro-GA9, in a cell-free system from bean, and conversion of 13-fluoro-GA9 into 13-fluoro-GA4 was also observed in a cell-free system from pumpkin. These results suggest that 13-fluoro-GA9 is one of the substrates of GA 3rβ-hydroxy-lases, and that 13-fluoro-GA9 is active as a result of the conversion to 13-fluoro-GA4 in rice and cucumber seedlings.

Keywords: Cucurbita maxima L.; Fluorogibberellin analogs; Gibberellin 3rβ-hydroxylase; Phaseolus vulgaris L.; Plant growth; Prohexadione

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.