Journal Article

Ascorbic Acid-Dependent Regulation of Redox Levels of Chlorogenic Acid and its Isomers in the Apoplast of Leaves of <i>Nicotiana tabacum</i> L.

Umeo Takahama

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 39, issue 7, pages 681-689
Published in print July 1998 | ISSN: 0032-0781
Published online July 1998 | e-ISSN: 1471-9053 | DOI: https://dx.doi.org/10.1093/oxfordjournals.pcp.a029422
Ascorbic Acid-Dependent Regulation of Redox Levels of Chlorogenic Acid and its Isomers in the Apoplast of Leaves of Nicotiana tabacum L.

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There is a question whether ascorbic acid (AA) can control redox levels of phenolics in the apoplast. The present study was designed to answer this question. AA, dehydroascorbic acid (DHA), chlorogenic acid (CGA) and its two structural isomers were present in the apoplast of leaves of tobacco (Nicotiana tabacum L. cv. BelW3). The levels of AA plus DHA (AA + DHA) and the ratios of AA to (AA + DHA) decreased while the levels of CGA plus its isomers increased during leaf aging. o-Quinones of CGA plus its isomers were found in the apoplast only in aged leaves of which apoplastic level of AA was nearly zero. In addition, activity of apoplastic peroxidase that could oxidize CGA and its isomers increased during leaf aging. From the observations, it is concluded that AA can regulate the accumulation of the o-quinones of CGA and its isomers in the apoplast. Based on the conclusion, it is proposed that soluble peroxidase in the apoplast has two functions, namely, (i) scavenging of H2O2 and/or regulation of the level of apoplastic H2O2 in the presence of AA, and (ii) accumulation of oxidation products of the phenolics in the absence of AA.

Keywords: Apoplast; Ascorbic acid; Chlorogenic acid and its isomers; Nicotiana tabacum; Peroxidase (EC 1.11.1.7); Regulation of redox level

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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