Journal Article

Crystalline Cellulose in Hydrated Primary Cell Walls of Three Monocotyledons and One Dicotyledon

Bronwen G. Smith, Philip J. Harris, Laurence D. Melton and Roger H. Newman

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 39, issue 7, pages 711-720
Published in print July 1998 | ISSN: 0032-0781
Published online July 1998 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029425
Crystalline Cellulose in Hydrated Primary Cell Walls of Three Monocotyledons and One Dicotyledon

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The molecular ordering of cellulose, including its crystallinity, in the unlignified primary cell walls of three monocotyledons (Italian ryegrass, pineapple, and onion) and one dicotyledon (cabbage) was characterized by solid-state 13C NMR spectroscopy. These species were chosen because their primary cell walls have different non-cellulosic polysaccharides and this may affect the molecular ordering of cellulose. Values of the proton rotating-frame relaxation [T1p(H)] and spin-spin relaxation [T2(H)] time constants showed that the cellulose in the cell walls of all four species was in a crystalline rather than an amorphous state. Furthermore, a resolution enhancement procedure showed that the triclinic (Iα) and the monoclinic (I) crystal forms of cellulose were present in similar proportions in these cell walls. However, the calculated cross-sectional dimensions of the cellulose crystallites varied among the cell walls (in the range 2–3 nm): the largest were in the Italian ryegrass, the smallest were in the onion and cabbage, and those of intermediate size were in the pineapple. The crystallite dimensions may thus be affected by the non-cellulosic polysaccha-ride compositions of the cell walls.

Keywords: Cellulose crystallites; Cell-wall polysaccharides; Monocotyledons; Non-cellulosic polysaccharides; Primary cell walls; Solid-state CP/MAS 13C NMR spectroscopy

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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