Journal Article

Salicylic Acid Induces Extracellular Superoxide Generation Followed by an Increase in Cytosolic Calcium Ion in Tobacco Suspension Culture: The Earliest Events in Salicylic Acid Signal Transduction

Tomonori Kawano, Nobuya Sahashi, Koji Takahashi, Nobuyuki Uozumi and Shoshi Muto

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 39, issue 7, pages 721-730
Published in print July 1998 | ISSN: 0032-0781
Published online July 1998 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029426
Salicylic Acid Induces Extracellular Superoxide Generation Followed by an Increase in Cytosolic Calcium Ion in Tobacco Suspension Culture: The Earliest Events in Salicylic Acid Signal Transduction

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Addition of salicylic acid (SA) to tobacco (Nicotiana tabacum) suspension culture immediately induced a rapid and transient generation of superoxide anion (O2), followed by a transient increase in cytosolic free calcium ion concentration ([Ca2+]c). The level of SA-induced O2 was lowered by treatment with several scavengers of active oxygen species and a peroxidase inhibitor, but not with an NADPH oxidase inhibitor. The SA-induced [Ca2+]c elevation was also lowered by inhibitors which effectively lowered the O2 level. Inhibition of [Ca2+]c elevation by Ca2+ channel blockers and a Ca2+ chelator indicated that extracellular Ca2+ was responsible for the increased [Ca2+]c. Among the several SA analogs, only compounds that actively induced the O2 generation also elevated [Ca2+]cIn addition, the inhibitory effects of SA analogs on catalase activity correlated well with their effects on the O2 generation and the [Ca2+]c elevation. SA-dependent O2 generation was shown to occur extracellularly, requiring both H2O2 and at least one proteinaceous factor excreted from the cells. This factor was determined to be a salicylhydroxamic acid-sensitive extracellular guaiacol-utilizing peroxidase.

Keywords: Cytosolic calcium; Extracellular peroxidase; Nicotiana tabacum; Salicylic acid; Signal transduction; Superoxide

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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