Journal Article

The Identification of DNA Binding Factors Specific for <i>as-I</i>-like Sequences in Auxin-Responsive Regions of <i>parA, parB</i> and <i>parC</i>

Tatsuya Sakai, Yohsuke Takahashi and Toshiyuki Nagata

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 39, issue 7, pages 731-739
Published in print July 1998 | ISSN: 0032-0781
Published online July 1998 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029427
The Identification of DNA Binding Factors Specific for as-I-like Sequences in Auxin-Responsive Regions of parA, parB and parC

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We have identified the auxin-responsive region (Aux-RR) of the par A promoter; it is derived from a gene that is induced by auxin in tobacco mesophyll protoplasts. By analyses of gain-of-function and point mutations in trans-genic tobacco plants, we showed that an as-1--like sequence was required, but not alone sufficient, for auxin responsiveness of the par A promoter, as has been also shown for the parC promoter in a recent study. A gel mobility shift assay revealed that the as-1-like sequence of the par A promoter bound specifically to the as-1 binding nuclear factor, ASF-1, while the as-1-like sequence of the parC promoter bound specifically to a novel nuclear factor named ALF-1 (as-1-like sequence binding factor-1). Since these bindings were correlated with auxin-induced activation of transcription, those results suggest that ALF-1 is a new nuclear factor involved in auxin responsiveness and that it is distinct from ASF-1. Furthermore, we found that an as-l-like sequence in the AuxRR of parB bound specifically to a tobacco nuclear factor, named ALF-2, which differed from ASF-1 and ALF-1. These results suggest that the auxin responsiveness of the auxin-inducible genes par A parB and parC are regulated by different mechanisms, even though the cis-acting elements look similar.

Keywords: as-1; ASF-1; Auxin-responsive region; Gene expression; Tobacco (Nicotiana tabacum)

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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