Journal Article

The Photorepair and Photoisomerization of DNA Lesions in Etiolated Cucumber Cotyledons after Irradiation by UV-B Depends on Wavelength

Yuichi Takeuchi, Mina Murakami, Nobuyoshi Nakajima, Noriaki Kondo and Osamu Nikaido

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 39, issue 7, pages 745-750
Published in print July 1998 | ISSN: 0032-0781
Published online July 1998 | e-ISSN: 1471-9053 | DOI: https://dx.doi.org/10.1093/oxfordjournals.pcp.a029429
The Photorepair and Photoisomerization of DNA Lesions in Etiolated Cucumber Cotyledons after Irradiation by UV-B Depends on Wavelength

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To examine the light-dependent repair of DNA photo-lesions, such as, cyclobutane pyrimidine dimers (CPDs) and pyrimidine-(6-4)-pyriinidone photoproducts [(6-4)pho-toproducts], we incubated cucumber cotyledons under monochromatic light (325-500 nm) after they had been exposed to UV-B irradiation. Irradiation at wavelengths between 375 and 425 nm was the most efficient for photo-repair of CPDs. The dependence on wavelength for the removal of (6-4)photoproducts differed from that for the photorepair of CPDs. The (6-4)photoproducts were most efficiently removed upon irradiation at 325 nm with a second peak at 400 to 425 nm. An immunological assay using antibodies specific to Dewar photoproducts revealed that irradiation at 325 nm induced the photoisomerization of (6-4)photoproducts to Dewar photoproducts. Thus, the most efficient wavelength for the photorepair of (6-4)photoprod-ucts was between 400 and 425 nm. It seems likely that processes similar to those involved in photorepair of CPDs might be involved in the photorepair of (6-4)photoprod-ucts.

Keywords: Cucumber; Cyclobutane pyrimidine dimer; Dewar photoproduct; DNA photorepair; Pyrimidine-(6-4); pyrimidone photoproduct

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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