Journal Article

Promoters of the Phycocyanin Gene Clusters of the Cyanobacterium <i>Synechococcus</i> sp. Strain PCC 7942

Hiromichi Sawaki, Tatsuo Sugiyama and Tatsuo Omata

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 39, issue 7, pages 756-761
Published in print July 1998 | ISSN: 0032-0781
Published online July 1998 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029431
Promoters of the Phycocyanin Gene Clusters of the Cyanobacterium Synechococcus sp. Strain PCC 7942

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The cyanobacterium Synechococcus sp. strain PCC 7942 has duplicated phycocyanin subunit gene clusters cpcB1A1 and cpcB2A2, which are identical to each other and to those of Synechococcus sp. strain PCC 6301 (Anacystis nidulans). Nucleotide sequences of the 428 and 286 bases of the 5′ non-coding regions of the cpcBlAl and cpcB2A2 clusters, respectively, of strain PCC 7942 were identical to those of strain PCC 6301. As in strain PCC 6301, cpcBlAl yielded two major transcripts of 1.4 and 1.3 kb and cpcB2A2 yielded a single transcript of 1.3 kb in strain PCC 7942. Thus, the structure and expression of cpcBA gene clusters in the two strains are essentially the same. Using bacterial luciferase encoded by luxAB as a reporter, cpcBlAl was shown to have two promoters corresponding to the two major transcripts. Luminescence from the Synechococcus reporter strains carrying the fusions of the cpcBA promoters to luxAB showed circadian oscillation. Similar to the promoter of psbA1 encoding the Dl protein of PSII, the two cpcBlAl promoters and the cpcB2A2 promoter showed the peak of activity at the end of the subjective day and the trough at the end of the subjective night.

Keywords: Circadian rhythm; Cyanobacterium; Phycocyanin; Promoter; Synechococcus; Transcription

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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