Journal Article

Mapping of 25 Drought-Inducible Genes, <i>RD</i> and <i>ERD</i>, in <i>Arabidopsis thaliana</i>

Teruaki Taji, Motoaki Seki, Kazuko Yamaguchi-Shinozaki, Hiroshi Kamada, Jérôme Giraudat and Kazuo Shinozaki

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 40, issue 1, pages 119-123
Published in print January 1999 | ISSN: 0032-0781
Published online January 1999 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029469
Mapping of 25 Drought-Inducible Genes, RD and ERD, in Arabidopsis thaliana

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We mapped 25 Arabidopsis thaliana drought-inducible genes, Responsive to Dehydration (RD) and Early Responsive to Dehydration (ERD), to the Arabidopsis genome and compared map positions with those of mutants that show environmental stress response. We hybridized CIC yeast artificial chromosome (YAC) library filters with the cDNAs and determined the map positions of 18 corresponding genes. We screened the P1 library with 7 other clones and analyzed segregation of their restriction fragment length polymorphisms (RFLP) in recombinant inbred (RI) lines. One cDNA could be mapped because it had been sequenced by the Arabidopsis genome sequencing program.

Keywords: Arabidopsis thaliana; Drought-inducible genes; Mapping; RFLP; YAC

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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