Journal Article

Expression of a Gene for Cyclophilin Which Contains an Amino-Terminal Endoplasmic Reticulum-Targeting Signal

Takeshi Saito, Yasuo Niwa, Hiroyuki Ashida, Katsunori Tanaka, Makoto Kawamukai, Hideyuki Matsuda and Tsuyoshi Nakagawa

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 40, issue 1, pages 77-87
Published in print January 1999 | ISSN: 0032-0781
Published online January 1999 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029477
Expression of a Gene for Cyclophilin Which Contains an Amino-Terminal Endoplasmic Reticulum-Targeting Signal

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  • Molecular and Cell Biology
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We isolated a novel gene for cyclophilin (CyP) first identified as an intracellular target of the immunosuppressant cyclosporin A and also known to have peptidyl-prolyl cis-trans isomerase (PPIase) activity, named ATCYP5 from Arabidopsis thaliana. ATCYP5 encoded a polypeptide with 201 amino acids with a putative ER-targeting signal sequence at its N-terminal, but without the typical ER-retention signal in its C-terminal. In addition, ATCYP5 protein contained a. seven amino-acid long sequence which has been found previously only in cytosolic CyPs from plants. The synthetic mutant green fluorescent protein (sGFP; S65T) was fused to the N-terminal part of ATCYPS, and expressed in tobacco BY-2 cells. The fluorescence derived from the fusion protein was detected mainly around the nucleus, indicating translocation into ER. ATCYP5 was expressed mainly in young stems especially in the apical region and weakly in leaves and roots.

Keywords: Arabidopsis thaliana; Cyclophilin; Endoplasmic reticulum; Green fluorescent protein; Peptidyl-prolyl cis-trans isomerase

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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