Journal Article

A Transgenic Mutant Defective in Cell Elongation and Cellular Organization during Both Root and Shoot Development in Lettuce, <i>Lactuca sativa</i>

Chang-Hsien Yang and Chuan-I Li

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 40, issue 11, pages 1108-1118
Published in print January 1999 | ISSN: 0032-0781
Published online January 1999 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029495
A Transgenic Mutant Defective in Cell Elongation and Cellular Organization during Both Root and Shoot Development in Lettuce, Lactuca sativa

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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A recessive mutation affecting both root and shoot development was isolated from transformants of lettuce cv. Diana co-transformed with maize Ac transposase and Ds. Mutant phenotype in the progeny was co-segregated with the T-DNA containing Ac transposase indicating that the mutation was caused by insertional mutagenesis. Mutation in this novel genetic locus, designated as ANORMAL ROOT AND SHOOT (ARS), showed a dwarf phenotype with short thick roots, short hypocotyl and abnormal filamentous leaves without any further reproductive development. The anatomical analysis revealed that the ars mutant root phenotype is primarily due to the lack of cell elongation and to the abnormal increase in cell number in the cortex region in the roots. ars mutants are able to initiate normal leaf primodia, but, the cell elongation and cellular organization of the developing leaf primodia is impaired and results in sequentially abnormal development of the leaf. ars mutants also display photomorphogenic development in darkness by producing open cotyledons, developing vegetative leaves, and short hypocotyls. This suggests that the ARS gene may also be involved in the regulation of cell elongation in the hypocotyl in the absence of light. Abnormal development in ars mutations can not be normalized by exogenous application of phytohormones such as gibberellin and brassinosteroids, indicating that the mutant is not impaired in the biosynthesis of these two hormones.

Keywords: ABNORMAL ROOT AND SHOOT (ARS); Cell elongation; Meristem; Photomorphogenesis; Root development; Shoot development

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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