Journal Article

Involvement of Cyclic AMP in ABA- and Ca<sub>2−</sub>-Mediated Signal Transduction of Stomatal Regulation in <i>Vicia faba</i>

Xiao-Chun Jin and Wei-Hua Wu

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 40, issue 11, pages 1127-1133
Published in print January 1999 | ISSN: 0032-0781
Published online January 1999 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029497
Involvement of Cyclic AMP in ABA- and Ca2−-Mediated Signal Transduction of Stomatal Regulation in Vicia faba

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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The focus of this study is to investigate possible involvement of cyclic AMP in regulation of Vicia stomatal movements. The presence of 0.1 mM 8-Br-cAMP, a membrane-permeable analogue of cAMP, alone in the incubation medium did not affect stomatal opening in the light in leaf epidermal peel experiments. However, addition of 0.1 mM 8-Br-cAMP completely reversed exogenous ABA- and Ca2+-induced inhibition of stomatal opening. Consistent with these results, patch-clamping experiments showed that intracellular addition of 0.5 mM or 1 mM cAMP significantly reversed the inhibition of whole-cell inward K+ currents by internally supplied 13 μM Ca2+ or 10 μM ABA in stomatal guard cell protoplasts, respectively. Furthermore, intracellular addition of either 10 μM prostaglandin E1 (PGE1, an adenylate cyclase activator) or 1 mM 3-isobutyl-1-methylxanthine (IBMX, a phosphodiesterase inhibitor) mimicked the effect of exogenous cAMP on the removal of ABA- or Ca2+ inhibition of inward K+-current. These results suggest that a cAMP signaling pathway is involved in signal transduction in stomatal regulation by interacting with ABA and Ca2+ signaling cascades. A hypothetical mechanism by which cAMP may regulate K+ in stomatal guard cells is also discussed.

Keywords: Abscisic acid; Cyclic AMP; Cytoplasmic Ca2+; K+-channel; Stomatal guard cells; Vicia faba

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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