Journal Article

Photosynthesis with Single-Rooted <i>Amaranthus</i> Leaves. I. Changes in the Activities of RuBP-1,5-bisphosphate Carboxylase and Phosphoenolpyruvate Carboxylase and the Amounts of Intermediates in Photosynthetic Metabolism in Response to Changes in the Source-Sink Balance

Shinichi Sawada, Osamu Arakawa, Isao Muraki, Hitoshi Echigo, Miyuki Miyashita, Mito Iwafune, Minobu Kasai and Hideaki Usuda

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 40, issue 11, pages 1143-1151
Published in print January 1999 | ISSN: 0032-0781
Published online January 1999 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029499
Photosynthesis with Single-Rooted Amaranthus Leaves. I. Changes in the Activities of RuBP-1,5-bisphosphate Carboxylase and Phosphoenolpyruvate Carboxylase and the Amounts of Intermediates in Photosynthetic Metabolism in Response to Changes in the Source-Sink Balance

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C4 model plants composed of single-rooted Amarantus cruentus L. leaves were developed to study source-sink relationships. The photosynthetic activity of CO4 fixation in the rooted leaves cultured under the control condition (10-h light/14-h darkness, 730 μmol photons m−2s−1) remained constant and high. When the leaves kept for 8 or 9 d under the control condition were exposed to continuous light (CL) for 4 d, the activity decreased steeply. The starch content increased significantly during the first 2 d in CL whereas the sucrose content increased almost linearly during the 4 d in CL. The close relationship between the decrease in photosynthetic activity and the increase in sucrose indicates that the activity was subjected to feedback inhibition when the end product was forced to accumulate in the leaves. In the treated leaves, the initial activity of ribulose-1,5-bisphoshate carboxylase decreased significantly. A large increase in ribulose-1,5-bisphosphate and decrease in 3-phosphoglycerate occurred following the deactivation in RuBPcase, which was fully restored by the preincubation with CO2 and Mg2+ and/or P1 and Mg2+. The activity of phosphoenolpyruvate carboxylase also decreased but not as rapidly as RuBPcase. The alanine and pyruvate levels decreased markedly but the oxaloacetate, glutamine and asparagine levels significantly increased while phosphoenolpyruvate and glutamate were kept at almost constant levels. The malate level first increased slightly and then decreased significantly. The mechanism of coordinated decrease in carboxylation reactions between phtosynthetic carbon reduction cycle and C4 metabolism in response to change in the source-sink balance is discussed.

Keywords: Amaranthus cruentus L.; C4 metabolism intermediates; Phosphoenolpyruvate carboxylase (EC 4.1.1.31); Ribulose-1,5-bisphoshate carboxylase; Single-rooted leaves; Source-sink C4 model plant

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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