Journal Article

Visualization of EXGT-Mediated Molecular Grafting Activity by Means of a Fluorescent-Labeled Xyloglucan Oligomer

Hisashi Ito and Kazuhiko Nishitani

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 40, issue 11, pages 1172-1176
Published in print January 1999 | ISSN: 0032-0781
Published online January 1999 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029503
Visualization of EXGT-Mediated Molecular Grafting Activity by Means of a Fluorescent-Labeled Xyloglucan Oligomer

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  • Biochemistry
  • Molecular and Cell Biology
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A fluoresceinyl xyloglucan heptasaccharide was synthesized and used as a probe to visualize the activity of the cell wall to incorporate xyloglucan fragments into the cellulose/xyloglucan framework in suspension-cultured tobacco cells. The activity was significantly reduced in transformant cells in which the expression of endoxyloglucan transferase (EXGT) was severely suppressed by over-expression of the antisense EXGT-NI mRNA. These results indicate the involvement of EXGT in the construction of the cellulose/xyloglucan framework.

Keywords: Antisense mRNA; Cell-wall construction; Cellulose/xyloglucan framework; Endoxyloglucan transferase; Fluoresceinyl xyloglucan oligomer; Tobacco BY-2 cell

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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