Journal Article

Localization of the Ca<sup>2+</sup>-Binding Protein, Bra r 1, in Anthers and Pollen Tubes

Takashi Okada, Zhaojie Zhang, Scott D. Russell and Kinya Toriyama

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 40, issue 12, pages 1243-1252
Published in print January 1999 | ISSN: 0032-0781
Published online January 1999 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029512
Localization of the Ca2+-Binding Protein, Bra r 1, in Anthers and Pollen Tubes

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Calcium plays an essential role during pollen development and pollen tube growth, and several Ca2+-binding protein are expressed in anthers. We have previously reported that Brassica pollen allergens encoded by Bra r 1 and Bra r 2 show sequence similarities to Ca2+-binding proteins [Toriyama et al. (1995) Plant Mol. Biol. 29: 1157]. Herein, we report that both genes are expressed in the diploid tapetum and haploid microspores, as detected by in situ RNA hybridization. Immunoblot analysis revealed that Bra r 1 and Bra r 2 were accumulated in anthers during pollen development. When pollen grains were suspended in an aqueous solution, both proteins were mainly detected in the pollen extracellular fraction, indicating that Bra r 1 and Bra r 2 are released from the pollen upon hydration. Localization of Bra r 1 was further investigated in sections of anthers and pollen tubes. Bra r 1 was detected in the tapetum, microspores and pollen grains. In longitudinal sections of cross-pollinated pistils, Bra r 1 was detected throughout pollen tubes elongating in transmitting-tissue. These findings suggest that Bra r 1 may be involved in pollen-pistil interaction and pollen tube growth.

Keywords: Calcium; Ca2+-binding protein; Immunolocalization; Pollen allergen; Pollen tube growth

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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