Journal Article

Developmental Transitions and Dynamics of the Cortical ER of <i>Arabidopsis</i> Cells Seen with Green Fluorescent Protein

Robert W. Ridge, Yoriko Uozumi, Jacek Plazinski, Ursula A. Hurley and Richard E. Williamson

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 40, issue 12, pages 1253-1261
Published in print January 1999 | ISSN: 0032-0781
Published online January 1999 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029513
Developmental Transitions and Dynamics of the Cortical ER of Arabidopsis Cells Seen with Green Fluorescent Protein

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Arabidopsis thaliana plants were stably transformed with DNA encoding green fluorescent protein and with sequences ensuring retention in the endoplasmic reticulum (ER). Confocal laser scanning microscopy shows fluorescent ER in many cells of seedlings so allowing developmental changes to be documented. The arrangement of the cortical ER changes as cells mature in the hypocotyl and root epidermis. In the root, cells that have completed expansion have reticulate cortical ER resembling the ER described in many previous studies. Expanding cells, however, show extensive perforated sheets of cortical ER which transform quite abruptly into a loose reticulum at the basipetal end of the elongation zone. The reticulum compacts in trichoblasts beginning at sites where root hairs are about to emerge. The compacted form is maintained throughout the hair until growth ceases and the open reticulate form returns. All forms of cortical ER are dynamic and we use a color overlay method to distinguish stable and moving structures in a single composite image. Reticulate ER continuously rearranges its polygonal layout and perforations move and change their shape in the ER sheets of younger cells. ER deeper in the cell (i.e. not close to the plasma membrane) moves more actively so that almost no tubules remain stable even over short periods of less than one minute. The function of the perforated sheets of cortical ER present in growing cells is unknown.

Keywords: Arabidopsis; Confocal laser scanning microscopy; Cortical endoplasmic reticulum; Green fluorescent protein; Motility; Root hairs

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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