Journal Article

Chloroplast NADH Dehydrogenase from <i>Pisum sativum</i>: Characterization of its Activity and Cloning of <i>ndhK</i> Gene

Felix Elortza, Juan A. Asturias and Jesús M. Arizmendi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 40, issue 2, pages 149-154
Published in print January 1999 | ISSN: 0032-0781
Published online January 1999 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029522
Chloroplast NADH Dehydrogenase from Pisum sativum: Characterization of its Activity and Cloning of ndhK Gene

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The pea chloroplast ndhK gene coding for a component of a NADH-plastoquinone oxidoreductase has been cloned and sequenced. This gene codes for a polypeptide of 227 amino acids and a predicted molecular mass of 25,495 Da which belongs to the family of the 20 kDa PSST subunit of the bovine mitochondrial complex I. A fragment of this gene has been overexpressed in Escherichia coli, and antibodies against the expressed polypeptide recognize a protein of the predicted molecular mass from pea thylakoid membranes. This polypeptide is a component of a protein complex with NADH dehydrogenase activity and is not associated with ferredoxin-NADP+reductase.

Keywords: Chlororespiration; Complex I; Cyclic electron transport; NAD(P)H dehydrogenase (EC 1.6.99.3); ndh gene; Pisum sativum

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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