Journal Article

Expression Characteristics of <i>CS-ACS1</i>, <i>CS-ACS2</i> and <i>CS-ACS3</i>, Three Members of the 1-Aminocyclopropane-1-Carboxylate Synthase Gene Family in Cucumber (<i>Cucumis sativus</i> L.) Fruit under Carbon Dioxide Stress

Francis M. Mathooko, Mercy W. Mwaniki, Akira Nakatsuka, Shinjiro Shiomi, Yasutaka Kubo, Akitsugu Inaba and Reinosuke Nakamura

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 40, issue 2, pages 164-172
Published in print January 1999 | ISSN: 0032-0781
Published online January 1999 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029524
Expression Characteristics of CS-ACS1, CS-ACS2 and CS-ACS3, Three Members of the 1-Aminocyclopropane-1-Carboxylate Synthase Gene Family in Cucumber (Cucumis sativus L.) Fruit under Carbon Dioxide Stress

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We investigated the expression pattern of three 1-aminocyclopropane-1-carboxylate (ACC) synthase genes, CS-ACS1, CS-ACS2 and CS-ACS3 in cucumber (Cucumis sativus L.) fruit under CO2 stress. CO2 stress-induced ethylene production paralleled the accumulation of only CS ACS1 transcripts which disappeared upon withdrawal of CO2 Cycloheximide inhibited the CO2 stress-induced ethylene production but superinduced the accumulation of CS-ACS1 transcript. At higher concentrations, cycloheximide also induced the accumulation of CS-ACS2 and CS-ACS3 transcripts. In the presence of CO2 and cycloheximide, the accumulation of CS-ACS2 transcript occurred within 1 h, disappeared after 3 h and increased greatly upon withdrawal of CO2 Inhibitors of protein kinase and types 1 and 2A protein phosphatases which inhibited and stimulated, respectively, CO2 stress-induced ethylene production had little effect on the expression of these genes. The results presented here identify CS-ACS1 as the main ACC synthase gene responsible for the increased ethylene biosynthesis in cucumber fruit under CO2 stress and suggest that this gene is a primary response gene and its expression is under negative control since it is expressed by treatment with cycloheximide. The results further suggest that the regulation of CO2 stress-induced ethylene biosynthesis by reversible protein phosphorylation does not result from enhanced ACC synthase transcription.

Keywords: ACC synthase; CO2 stress; Cucumber; Cucumis sativus; Ethylene biosynthesis; Gene expression; Signal transduction

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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