Journal Article

Two mRNA Species Encoding Calcium-Dependent Protein Kinases Are Differentially Expressed in Sexual Organs of <i>Marchantia polymorpha</i> through Alternative Splicing

Rie Nishiyama, Hiroshi Mizuno, Sachiko Okada, Tomoya Yamaguchi, Mizuki Takenaka, Hideya Fukuzawa and Kanji Ohyama

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 40, issue 2, pages 205-212
Published in print January 1999 | ISSN: 0032-0781
Published online January 1999 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029529
Two mRNA Species Encoding Calcium-Dependent Protein Kinases Are Differentially Expressed in Sexual Organs of Marchantia polymorpha through Alternative Splicing

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  • Biochemistry
  • Molecular and Cell Biology
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In plants, calcium-dependent calmodulin-independent protein kinases (CDPKs) are the predominant calcium- regulated protein kinases and their genes are encoded by a multigene family. A CDPK gene was cloned from a liverwort, Marchantia polymorpha, which showed a high level of sequence similarities to other higher plant CDPK genes. The liverwort CDPK gene consisted of 9 exons and 8 introns. The 6th and 7th exons (Exon 6A and Exon 6B) were almost identical except for 4-amino acid substitutions, both of which coded for EF-hands in the calciumbinding domain. RT-PCR analysis revealed that two species of mature mRNA containing either Exon 6A or Exon 6B were generated from a single CDPK gene by mutually exclusive alternative splicing. Both histidine-tagged fusion proteins derived from cDNAs containing either Exon 6A or Exon 6B exhibited calcium-dependent protein kinase activity in vitro. Preferential accumulation of the mature mRNA with Exon 6A detected in male sexual organ implies possible sexual control of the ratio between the two CDPK isozymes through alternative splicing. Functions and evolution of CDPKs are discussed based on the structure and expression of the liverwort CDPK gene.

Keywords: Alternative splicing; Calcium-dependent protein kinase; Liverwort; Plant; Sexual organs

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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