Journal Article

A Wheat Cold-Regulated cDNA Encoding an Early Light-Inducible Protein (ELIP): Its Structure, Expression and Chromosomal Location

Etsuo Shimosaka, Tsuneo Sasanuma and Hirokazu Handa

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 40, issue 3, pages 319-325
Published in print January 1999 | ISSN: 0032-0781
Published online January 1999 | e-ISSN: 1471-9053 | DOI: https://dx.doi.org/10.1093/oxfordjournals.pcp.a029544
A Wheat Cold-Regulated cDNA Encoding an Early Light-Inducible Protein (ELIP): Its Structure, Expression and Chromosomal Location

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Sequencing and computer analysis revealed that a wcr (wheat cold-regulated) cDNA, wcr12, contained a 525 bp open reading frame encoding a polypeptide of 174 amino acids. The deduced WCR12 protein had a high sequence similarity with Early light-inducible proteins (ELIPs) which are known as nuclear-encoded chloroplast proteins induced by light stress. The accumulation of wcr12 transcripts was induced by low temperature below 8°C and was also increased under a high light flux of 1,000 μEm−2s−1, which are typical expression patterns of the genes for ELIPs in other plants. The dramatic increase in the level of wcrl2 transcripts was detected at 4°C even under a low light condition (50 μEm−2s−1) and the level of transcripts became 20-fold higher than the control at 20°C under a moderate light condition (250 μEm−2s−1. Surprisingly, the accumulation of wcrl2 mRNAs was also induced by cold treatment alone at 4°C in the darkness. The level of wcrl2 transcripts accumulated in the darkness at 4°C reached the same level as that under a high light flux at 20°C. These results indicate that light is not essential for the accumulation of wcrl2 transcripts at a low temperature. To determine the chromosomal location of wcrl2 gene, we performed Southern analysis using the nullitetrasomic lines. The data showed that the wcrl2 gene or related homologues are located on the chromosomes of homoeologous group-5 in wheat.

Keywords: Cold-regulated gene; ELIPs; Photoinhibition; Stress induction; Wheat

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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