Journal Article

Localization of Farnesyl Diphosphate Synthase in Chloroplasts

Kazutsuka Sanmiya, Osamu Ueno, Makoto Matsuoka and Naoki Yamamoto

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 40, issue 3, pages 348-354
Published in print January 1999 | ISSN: 0032-0781
Published online January 1999 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029549
Localization of Farnesyl Diphosphate Synthase in Chloroplasts

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The subcellular localization of plant farnesyl diphosphate synthase (FPPS) was examined. Immunocytochemical staining using anti-FPPS1 antibody followed by electron microscopy showed that FPPS1 was localized to chloroplasts of rice mesophyll cells. Subcellular fractions from wheat leaves were examined by immunoblot analysis. FPPS was detected in the chloroplast fraction in wheat, and was protected from proteolysis following trypsin treatment of chloroplasts. FPPS was also detected in the chloroplast fraction of a dicot plant, tobacco.

Keywords: Chloroplast; Farnesyl diphosphate synthase (EC 2.5.l.l/EC 2.5.1.10); Isoprenoid; Rice (Orizasativa L.); Subcellular localization

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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