Journal Article

Light-Regulated Expression of the Gene for C<sub>4</sub>-Form Phospho<i>enol</i>pyruvate Carboxylase in Maize: Destabilization of mRNA by Okadaic Acid

Ken-ichi Kurotani, Shingo Hata and Katsura Izui

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 40, issue 4, pages 423-430
Published in print January 1999 | ISSN: 0032-0781
Published online January 1999 | e-ISSN: 1471-9053 | DOI: https://dx.doi.org/10.1093/oxfordjournals.pcp.a029558
Light-Regulated Expression of the Gene for C4-Form Phosphoenolpyruvate Carboxylase in Maize: Destabilization of mRNA by Okadaic Acid

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In Zea mays the transcripts of the phosphoenolpyruvate carboxylase (PEPC) gene for C4 photosynthesis (Ppc1A) is known to be accumulated during greening of etiolated leaves in response to light. Effects of several kinds of inhibitors on this light-induction were investigated. The accumulation of Ppc1A mRNA was almost completely inhibited by 0.1 μM okadaic acid (OA), a protein phosphatase (PP) 1 and PP2A inhibitor as revealed by northern blot analysis. In contrast, staurosporine (ST) and K252a, protein kinase inhibitors, were not effective. Diurnal change of Ppc1A mRNA level in green leaves was also collapsed by 0.1 μM OA. Accumulation of the transcripts of light harvesting chlorophyll a/b binding protein genes (Cab) during greening was also inhibited by OA, but that of the gene for a non-photosynthetic C3-form PEPC (Ppc1B) was not. Run-on transcription analysis showed that though the transcriptional rates of Ppc1A and Cab were increased by exposure of etiolated seedlings to light, their rates were not affected by 1 μM OA. Taken together, it was proposed that a certain PP is involved in the high-level accumulation of Ppc1A mRNA under light condition through stabilization of its transcripts.

Keywords: C4-photosynthesis; mRNA stability; Okadaic acid; Phosphoenolpyruvate carboxylase (EC 4.1.1.31); Zea mays

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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