Journal Article

Molecular Cloning and Characterization of a cDNA Encoding Caltractin from <i>Dunaliella salina</i>

Jae Heung Ko, Jae Yoon Kim and Sun Hi Lee

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 40, issue 4, pages 457-461
Published in print January 1999 | ISSN: 0032-0781
Published online January 1999 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029564
Molecular Cloning and Characterization of a cDNA Encoding Caltractin from Dunaliella salina

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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We cloned a cDNA encoding caltractin, a 20 kDa calcium-binding protein, from Dunaliella salina (DSCALT). The Ca2+-bound mobility shift detected in Chlamydomonas caltractin was hardly detectable in DSCALT. Also, some differences were found in the electrophoretic mobility between the native DSCALT and the bacterial-expressed DSCALT. This difference may have resulted from the posttranslational modification. Immunoblot analysis revealed that this protein might be localized mainly in the basal body complex, the major microtubule organizing center (MTOC) in D. salina and the functional homologue of the centrosome in the animal cell.

Keywords: Basal body; Caltractin; cDNA cloning; Dunaliella salina

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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