Journal Article

The Structure and Organization of Two Cysteine Endopeptidase Genes from Rice

Hideki Kato, Ai Shintani and Takao Minamikawa

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 40, issue 4, pages 462-467
Published in print January 1999 | ISSN: 0032-0781
Published online January 1999 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029565
The Structure and Organization of Two Cysteine Endopeptidase Genes from Rice

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

REP-1 is a major cysteine endopeptidase that digests seed storage glutelin of rice. A cDNA clone (pRP60) for REP-1 and a cDNA clone (pRP80) for a related enzyme were previously isolated. The expression of both mRNAs is regulated by gibberellin. In this study, we revealed the structure and organization of Rep1 and RepA, genes corresponding to pRP60 and pRP80 mRNAs, respectively. Rep1 has no introns whereas RepA consists of five exons and four introns, and both genes exist as one copy gene in the rice genome. The gibberellin-responsive elements conserved in cereal α-amylase genes are not included in the 5′-upstream region of Rep1 or RepA. A molecular phylogenetic tree of plant cysteine endopeptidases was constructed, and their relationship was discussed.

Keywords: Cysteine endopeptidase; Gibberellic acid; Nucleotide sequences; Oryza sativa; Rice seed

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.