Journal Article

Molecular Cloning and Expression of <i>Arabidopsis</i> Fatty Acid Hydroperoxide Lyase

Kenji Matsui, Jack Wilkinson, Bill Hiatt, Vic Knauf and Tadahiko Kajiwara

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 40, issue 5, pages 477-481
Published in print January 1999 | ISSN: 0032-0781
Published online January 1999 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029567
Molecular Cloning and Expression of Arabidopsis Fatty Acid Hydroperoxide Lyase

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  • Biochemistry
  • Molecular and Cell Biology
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Fatty acid hydroperoxide lyase (HPOL), an enzyme of the octadecanoid pathway that forms carbon-6 aldehydes such as n-hexanal or (Z)-3-hexenal, was cloned from Arabidopsis thaliana as a full-length cDNA. The HPOL activity obtained by expressing the cDNA in Escherichia coli formed n-hexanal from linoleic acid 13-hydroperoxide, whereas linoleic acid 9-hydroperoxide was not a substrate for the enzyme. The HPOL mRNA is expressed at low level in leaves; however, its accumulation can be found in the inflorescence. Wounding or methyl jasmonate treatments increase the mRNA level in leaves. These results indicate that the HPOL gene is up-regulated in leaves in response to wounding and that the enzyme may be an active component of the octadecanoid defense response.

Keywords: Arabidopsis; Fatty acid hydroperoxide lyase; (Z)-3-hexenal

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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