Journal Article

Overproduction of <i>Arabidopsis thaliana</i> FeSOD Confers Oxidative Stress Tolerance to Transgenic Maize

Frank Van Breusegem, Luit Slooten, Jean-Marie Stassart, Tanya Moens, Johan Botterman, Marc Van Montagu and Dirk Inzé

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 40, issue 5, pages 515-523
Published in print January 1999 | ISSN: 0032-0781
Published online January 1999 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029572
Overproduction of Arabidopsis thaliana FeSOD Confers Oxidative Stress Tolerance to Transgenic Maize

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Transgenic maize (Zea mays L.) plants have been generated by particle gun bombardment that overproduce an Arabidopsis thaliana iron superoxide dismutase (FeSOD). To target this enzyme into chloroplasts, the mature Fesod coding sequence was fused to a chloroplast transit peptide from a pea ribulose-1,5-bisphosphate carboxylase gene. Expression of the chimeric gene was driven by the CaMV 35S promoter. Growth characteristics and in vitro oxidative stress tolerance of transgenic lines grown in control and chilling temperatures were evaluated. The transgenic line with the highest transgenic FeSOD activities had enhanced tolerance toward methyl viologen and had increased growth rates.

Keywords: Chilling; Methyl viologen; Oxidative stress; Superoxide dismutase (EC 1.15.1.1); Transgenic plant; Zea mays

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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