Journal Article

Structural and Immunological Characterization of the <i>Cuscuta pentagona</i> L. Chloroplast

Timothy D. Sherman, William T. Pettigrew and Kevin C. Vaughn

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 40, issue 6, pages 592-603
Published in print January 1999 | ISSN: 0032-0781
Published online January 1999 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029582
Structural and Immunological Characterization of the Cuscuta pentagona L. Chloroplast

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Structural and immunochemical studies were used to determine the photosynthetic potential of the dodder (Cuscuta pentagona) chloroplast. Ultrastructural studies revealed that thylakoid membranes of pre-parasitic phase Cuscuta pentagona are almost all organized into long, overlapping grana stacks of mainly two to five thylakoids with little space between adjacent stacks. Immunoblots reveal chloroplast proteins associated with PSI and II, as well as cytochrome f and plastocyanin. Stromal extracts contained immmunologically-detectable RuBisCO and phosphoribulokinase. Cytochemical localizations of the oxidizing side of PSI showed product localization on the lumen side of the thylakoid. Immunocytochemical localizations of RuBisCO reveal exclusive labeling in the stroma, whereas antibodies to the PSII proteins, light-harvesting Chl a/b complex and the oxygen-evolving complex of PSII, are concentrated over the thylakoids. A limited capacity for CO2 fixation was found in seedlings by monitoring CO2 exchange rates in the presence and absence of atrazine. These data indicate that the chloroplast from this species of dodder contains a number of the proteins required for a successful fixation of CO2 and the proteins in the thylakoids are organized much like other higher plants, with the exception of the large percentage of the thylakoids organized into grana.

Keywords: Chloroplasts; Cuscuta pentagona; Dodder; Immunological characterization; Parasitic.plants; Ultrastructure

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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