Journal Article

Expression of the Auxin-Inducible GH3 Promoter/GUS Fusion Gene as a Useful Molecular Marker for Auxin Physiology<sup>1</sup>

Yi Li, Yan H. Wu, Gretchen Hagen and Tom Guilfoyle

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 40, issue 7, pages 675-682
Published in print January 1999 | ISSN: 0032-0781
Published online January 1999 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029592
Expression of the Auxin-Inducible GH3 Promoter/GUS Fusion Gene as a Useful Molecular Marker for Auxin Physiology1

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Expression of the GH3 promoter/GUS reporter (GH3/GUS) gene in transgenic tobacco is silent in most vegetative tissues and organs, but can be specifically induced by auxin in all cell types throughout the plant, indicating that auxin is a limiting factor for activation of the GH3/GUS gene. In the absence of exogenous auxin, expression of the GH3/GUS gene either correlates with physiological events which are presumably mediated by auxin or occurs in cells where relatively high levels of endogenous auxin are expected. For example, the GH3/GUS gene is expressed in dormant lateral buds, tips of adventitious roots, and stem parenchyma cells where adventitious roots are initiating. The GH3/GUS gene is activated at the cut surface nearest the tip of a leaf from which a strip of tissue has been excised, and in the basal end of an excised shoot segment. Also, the GH3/GUS gene is expressed predominantly in the lower side of a gravistimulated shoot and in the dark side of a unilateral light-stimulated shoot. Furthermore, the expression pattern of the GH3/GUS gene is similar to the distribution pattern of 14C-IAA that is applied at the apical end of the gravistimulated shoot. Thus, the results reported here provide additional support for a crucial role of auxin in shoot gravi- and photo-tropisms and adventitious root formation. Furthermore, the data presented in this report also indicate that expression of the GH3/GUS gene is an excellent molecular marker for studying auxin transport and for monitoring changes in either auxin concentration or cellular sensitivity to auxin in planta.

Keywords: Auxin; GH3 gene; Gene expression; Molecular marker

Journal Article.  0 words. 

Subjects: Plant Sciences and Forestry ; Biochemistry ; Molecular and Cell Biology

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