Journal Article

Differentially and Developmentally Regulated Expression of Three Rice Sucrose Synthase Genes

Ai-Yu Wang, Man-Han Kao, Wei-Horng Yang, Yiyang Sayion, Li-Fei Liu, Ping-Du Lee and Jong-Ching Su

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 40, issue 8, pages 800-807
Published in print January 1999 | ISSN: 0032-0781
Published online January 1999 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029608
Differentially and Developmentally Regulated Expression of Three Rice Sucrose Synthase Genes

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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The spatial and temporal distribution of sucrose synthase (RSuS) in rice (Oryza sativa L.) was studied by Western and immunohistochemical analyses using the mono-specific antibodies for three RSuS isoforms. In leaf tissues, RSuS1 was localized in the mesophyll while RSuS2 was in the phloem in addition to the mesophyll. In the roots, only RSuS1 was found in the phloem. No RSuS3 could be detected in any parts of etiolated seedlings. The expression of each RSus gene is closely linked to the seed development. RSuS1 was present in the aleurone layers of developing seeds, and at a low level in endosperm cells. RSuS2 was evenly distributed in seed tissues other than the endosperm. RSuS3 was localized predominantly in the endosperm cells. The tissue specific localizations of the three gene products suggest that RSuS1 plays a role in sugar transport into endosperm cells where the reaction catalyzed by RSuS3 provides the precursor of starch synthesis. RSus2, which is ubiquitously expressed, may play a housekeeping role.

Keywords: Gene expression; Immunohistochemistry; Monospecific antibodies; Oryza sativa; Rice; Sucrose synthase (EC 2.4.1.13)

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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