Journal Article

Molecular Cloning of Vacuolar H<sup>+</sup>-pyrophosphatase and Its Expression during the Development of Pear Fruit

Yasuo Suzuki, Masayoshi Maeshima and Shohei Yamaki

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 40, issue 8, pages 900-904
Published in print January 1999 | ISSN: 0032-0781
Published online January 1999 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029620
Molecular Cloning of Vacuolar H+-pyrophosphatase and Its Expression during the Development of Pear Fruit

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The cDNA of vacuolar proton-translocating inorganic pyrophosphatase was cloned from pear fruit. It contained an open reading frame of 2,301 nucleotides, coding for a polypeptide of 767 amino acids. The level of mRNA was very low in young fruit and increased with maturation, differing from the changes in the level of polypeptide.

Keywords: cDNA cloning; Fruit development; Pear (Pyrus communis); Vacuolar H+ pyrophosphatase (EC 3.6.1.1)

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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