Journal Article

In Vivo and in Vitro Conversion of Teasterone to Typhasterol in Cultured Cells of <i>Marchantia polymorpha</i>

Seung-Hyun Park, Kwang-Seok Han, Tae-Wuk Kim, Jae-Kuk Shim, Suguru Takatsuto, Takao Yokota and Seong-Ki Kim

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 40, issue 9, pages 955-960
Published in print January 1999 | ISSN: 0032-0781
Published online January 1999 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029628
In Vivo and in Vitro Conversion of Teasterone to Typhasterol in Cultured Cells of Marchantia polymorpha

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Identification by full-scan GC-MS revealed that [2H6]-teasterone fed to suspension cultured cells of Marchantia polymorpha was converted to [2H6]3-dehydroteasterone and [2H6]typhasterol. This indicates that the cells carry out a C3-epimerization in which teasterone is converted to typhasterol via 3-dehydroteasterone. In vitro enzymatic conversions of teasterone to typhasterol were also investigated. A crude cytosolic solution prepared from Marchantia cells catalyzed not only the dehydrogenation of teasterone to 3-dehydroteasterone but also the reduction of 3-dehydroteasterone to typhasterol. The major 4-demethysterol in cultured M. polymorpha cells was 24-methylcholesterol, the precursor of brassinosteroids. These results suggest that enzymes similar to those involved in the early C-6 oxidation pathway of the brassinosteroid biosynthesis are present in the liverwort.

Keywords: Biosynthesis; Brassinosteroids; C3-epimerization; 3-Dehydroteasterone reductase; Marchantia polymorpha; Teasterone dehydrogenase

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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