Journal Article

Induction of Defense Responses by Synthetic Glycopeptides that Have a Partial Structure of the Elicitor in the Spore Germination Fluid of <i>Mycosphaerella pinodes</i>

Akinori Kiba, Tadahiro Takeda, Takuya Kanemitsu, Kazuhiro Toyoda, Yuki Ichinose, Tetsuji Yamada and Tomonori Shiraishi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 40, issue 9, pages 978-985
Published in print January 1999 | ISSN: 0032-0781
Published online January 1999 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a029631
Induction of Defense Responses by Synthetic Glycopeptides that Have a Partial Structure of the Elicitor in the Spore Germination Fluid of Mycosphaerella pinodes

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A high molecular weight elicitor (>70 kDa) from spore germination fluid of a pea pathogen, Mycosphaerella pinodes, has a partial structure of β-D-Glc-(1 → 6)-α-D-Man-(1 → 6)-D-Man, which is O-glycosidically attached to serine in the protein moiety. To elucidate the minimum structure for the elicitor activity to pea plants, the effects of nine glycopeptides including β-D-Glc-(1 →÷ 6)-α-D-Man- (1 → 6)-n-Man-O-Ser (No. 1) to [β-D-Glc-(1 →6)-α-D-Man- (1 → 6)-D-Man]3 (No. 9) on the infection by M. pinodes, superoxide generation and ATPase activity were measured. The glycopeptides [β-D-Glc-(1 → 6)-α-D- Man-(1 → 6)-D-Man]-O-Ser2-Pro2 (No. 3) to No. 9 induced rejection reaction of pea tissue against M.pinodes. The glycopeptides No. 3 to No. 9 also induced superoxide generation on uninjured pea leaves. Moreover, the glycopeptides No. 3 to No. 9 induced in vitro the activation of cell wall-bound ATPase and superoxide generation system in the protein fraction solubilized from pea cell wall. The results indicate that the synthetic glycopeptides, No. 3 to No. 9, are available to analyze the signal transduction cascade leading to defense responses and the receptor for the elicitor.

Keywords: Cell wall-bound ATPase; Synthetic glycopeptide elicitor; Mycosphaerella pinodes; Pisum sativum L.; Rejection reaction; Superoxide generation

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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