Journal Article

cDNA Cloning of Sesquiterpene Cyclase and Squalene Synthase, and Expression of the Genes in Potato Tuber Infected with <i>Phytophthora infestans</i>

Hirofumi Yoshioka, Naofumi Yamada and Noriyuki Doke

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 40, issue 9, pages 993-998
Published in print January 1999 | ISSN: 0032-0781
Published online January 1999 | e-ISSN: 1471-9053 | DOI: https://dx.doi.org/10.1093/oxfordjournals.pcp.a029633
cDNA Cloning of Sesquiterpene Cyclase and Squalene Synthase, and Expression of the Genes in Potato Tuber Infected with Phytophthora infestans

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  • Biochemistry
  • Molecular and Cell Biology
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Sesquiterpene cyclase and squalene synthase are key branch point enzymes in isoprenoid pathway for the synthesis of sesquiterpenoid phytoalexins and sterols/steroid glycoalkaloids, respectively. cDNA clones encoding these enzymes were isolated from potato. A phylogenetic tree showed that the sesquiterpene cyclase is vetispiradiene synthase. Infection of Phytophthora infestans with potato tubers caused transient increases in the transcript level of vetispiradiene synthase in a compatible and an incompatible interactions. On the other hand, wound expression of the squalene synthase was suppressed in favor of the expression of vetispiradiene synthase regardless of inoculated races.

Keywords: Defense gene; Phytoalexin; Phytophthora infestans; Potato tuber; Sesquiterpene cyclase; Squalene synthase (EC 2.5.1.21)

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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