Journal Article

Mutation Induction in Human Cells after Low Dose X Ray Exposure

F. Yatagai, S. Morimoto, S. Goto, T. Kato, M. Honma and F. Hanaoka

in Radiation Protection Dosimetry

Volume 99, issue 1-4, pages 241-243
Published in print June 2002 | ISSN: 0144-8420
Published online June 2002 | e-ISSN: 1742-3406 | DOI: http://dx.doi.org/10.1093/oxfordjournals.rpd.a006773
Mutation Induction in Human Cells after Low Dose X Ray Exposure

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Mutations induced after low dose ionising radiation exposure have been intensively analysed not only for radiation risk estimation but also for basic understanding of cellular responses. Human lymphoblastoid TK6-20C cells were irradiated with 100 mGy of X rays and mutation at the heterozygous thymidine kinase (TK) locus was selected by trifluorothymidine resistance. Although the mutation frequency at the TK locus increased from 5.6 x 10-6 to 7.4 x 10-6, this increase was not statistically significant. However, molecular analysis of TK mutants exhibiting loss of heterozygocity (LOH) demonstrated a clear effect of such low dose IR exposure. Exposure to 100 mGy X ray increased the fraction of hemizygous-LOH from 10% to 42%. In previous experiments, a similar tendency in the increase of hemizygous-LOH was also observed in TK6 cells after exposure to a 2 Gy dose of X rays. This type of LOH can be considered as a result of end-joining repair of DNA double strand breaks.

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Subjects: Nuclear Chemistry, Photochemistry, and Radiation

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