Journal Article

Proto-Oncogene Expression: A Predictive Assay for Radiation Biodosimetry Applications

A. C. Miller, L. Luo, W. K. Chin, A. E. Director-Myska, P. G. S. Prasanna and W. F. Blakely (INVITED)

in Radiation Protection Dosimetry

Volume 99, issue 1-4, pages 295-302
Published in print June 2002 | ISSN: 0144-8420
Published online June 2002 | e-ISSN: 1742-3406 | DOI: http://dx.doi.org/10.1093/oxfordjournals.rpd.a006789
Proto-Oncogene Expression: A Predictive Assay for Radiation Biodosimetry Applications

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Using a model system of in vitro human peripheral blood lymphocytes, the effect of low-dose (0.25 to 1.50 Gy) 250-kVp X ray radiation (1 Gy.min-1) on the expression of several proto-oncogenes was examined (c-Haras, c-src, c-met, c-jun, c-fos, and c-myc) and ß-actin from 0.25 to 17 h post-radiation. RNA was extracted from cells harvested at various times after exposure and examined for levels of particular mRNAs by northern blot hybridisation. A progressive time- and dose-dependent increase in mRNA levels was observed for c-Haras mRNA, while the other proto-oncogenes (c-src, c-met, c-fos, c-jun, and c-myc) examined were variable during the same time period. ß-actin levels were initially decreased but at 17 h post-radiation had returned to control levels. A comparison of the rate of c-Haras transcription at 5 and 17 h post-irradiation revealed that c-Haras transcription was higher at 5 h than at 17 h. These findings suggest that the level of specific proto-oncogene expression, particularly c-Haras, may be useful early diagnostic molecular biomarkers for biodosimetry applications. The use of real-time PCR technologies to quantify gene expression changes will also be discussed.

Journal Article.  0 words. 

Subjects: Nuclear Chemistry, Photochemistry, and Radiation

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