Journal Article

Symbiotic Rhizobium and Nitric Oxide Induce Gene Expression of Non-symbiotic Hemoglobin in <i>Lotus japonicus</i>

Yoshikazu Shimoda, Maki Nagata, Akihiro Suzuki, Mikiko Abe, Shusei Sato, Tomohiko Kato, Satoshi Tabata, Shiro Higashi and Toshiki Uchiumi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 46, issue 1, pages 99-107
Published in print January 2005 | ISSN: 0032-0781
Published online January 2005 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pci/pci001
Symbiotic Rhizobium and Nitric Oxide Induce Gene Expression of Non-symbiotic Hemoglobin in Lotus japonicus

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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We characterized the expression profiles of LjHb1 and LjHb2, non-symbiotic hemoglobin (non-sym-Hb) genes of Lotus japonicus. Although LjHb1 and LjHb2 showed 77% homology in their cDNA sequences, LjHb2 is located in a unique position in the phylogenetic tree of plant Hbs. The 5′-upstream regions of both genes contain the motif AAAGGG at a position similar to that in promoters of other non-sym-Hb genes. Expression profiles obtained by using quantitative RT–PCR showed that LjHb1 and LjHb2 were expressed in all tissues of mature plants, and expression was enhanced in mature root nodules. LjHb1 was strongly induced under both hypoxic and cold conditions, and by the application of nitric oxide (NO) donor, whereas LjHb2 was induced only by the application of sucrose. LjHb1 was also induced transiently by the inoculation with the symbiotic rhizobium Mesorhizobium loti MAFF303099. Observations using fluorescence microscopy revealed the induction of LjHb1 expression corresponded to the generation of NO. These results suggest that non-sym-Hb and NO have important roles in stress adaptation and in the early stage of legume–rhizobium symbiosis.

Keywords: Keywords: Hypoxia — Lotus japonicus — Nitric oxide — Non-symbiotic hemoglobin — Plant hormone — Rhizobium.; Abbreviations: BA, 6-benzylaminopurine; c-PTIO, carboxy-2-phenyl-4,4,5,5-tetramethylimidazoline-3-oxide-1-oxyl; DAF-FM, 4-amino-5-methylamino-2′,7′-difluorofluorescein; GA3, gibberellic acid; Hb, hemoglobin; NO, nitric oxide; non-sym-Hb, non-symbiotic hemoglobin; RT–PCR, reverse transcriptase–polymerase chain reaction; SNAP, S-nitroso-N-acetyl-d,l-penicillamine; sym-Hb, symbiotic hemoglobin.

Journal Article.  5589 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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