Journal Article

Role of Malate Synthesis Mediated by Phosphoenolpyruvate Carboxylase in Guard Cells in the Regulation of Stomatal Movement

Naoko Asai, Nobuyoshi Nakajima, Masanori Tamaoki, Hiroshi Kamada and Noriaki Kondo

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 41, issue 1, pages 10-15
Published in print January 2000 | ISSN: 0032-0781
Published online January 2000 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/41.1.10
Role of Malate Synthesis Mediated by Phosphoenolpyruvate Carboxylase in Guard Cells in the Regulation of Stomatal Movement

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To clarify the pathway and role of malate synthesis in guard cells, epidermal strips isolated from Vicia faba L. leaflets were treated with 3,3-dichloro-2-dihydroxyphosphinoylmethyl-2-propenoate (DCDP), a specific inhibitor of phosphoenolpyruvate carboxylase (PEPC). When darkclosed stomata were illuminated, malate accumulated in guard cells and stomata opened; these were inhibited by 60% and 30%, respectively, by 5 mM DCDP treatment. When light-opened stomata were treated with DCDP, both malate level in guard cells and stomatal aperture decreased. Treatment with 5 mM DCDP partially inhibited CO2 incorporation into malate in guard cells. Treatment with mannitol at 0.4 M (osmotic stress) in the light increased malate level in guard cells and closed stomata. DCDP treatment decreased both malate level and stomatal aperture under stressed condition. These results show that malate synthesis in the light under both non-stressed and stressed conditions is dependent on PEPC activity. The extent of the decrease in malate level by DCDP treatment was larger under stressed condition than under nonstressed condition, suggesting that osmotic stress may enhance the activity of this pathway of malate synthesis which is induced by light. Role of malate synthesis in guard cells is discussed.

Keywords: DCDP; Guard cell; Malate; Osmotic stress; Phosphoenolpyruvate carboxylase; Vicia faba

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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