Journal Article

A Secreted Peptide Growth Factor, Phytosulfokine, Acting as a Stimulatory Factor of Carrot Somatic Embryo Formation

Hidetoshi Hanai, Takuya Matsuno, Manabu Yamamoto, Yoshikatsu Matsubayashi, Toshihiro Kobayashi, Hiroshi Kamada and Youji Sakagami

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 41, issue 1, pages 27-32
Published in print January 2000 | ISSN: 0032-0781
Published online January 2000 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/41.1.27
A Secreted Peptide Growth Factor, Phytosulfokine, Acting as a Stimulatory Factor of Carrot Somatic Embryo Formation

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Somatic embryogenesis of the carrot (Daucus carota L.) depends on a set of factors, some of which accumulate in culture medium (conditioned medium, CM). When embryogenic cell clusters were transferred to an embryo-inducing medium, addition of CM derived from somatic embryo culture markedly stimulated somatic embryo formation. The active principles were purified using a simple bioassay system and identified to be phytosulfokines (PSKs), sulfated oligopeptide growth factors originally isolated from a CM derived from asparagus (Asparagus officinalis L.) mesophyll culture. Quantification studies using a competition ELISA system employing an anti-PSK-α polyclonal antibody showed that PSK production might be related to growth of cells, rather than development of somatic embryos. Thus the stimulatory effect of PSK on somatic embryo formation might be due to promotion of cell proliferation.

Keywords: Daucus; Phytosulfokine-α; Somatic embryogenesis; Sulfated peptide

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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