Journal Article

Accumulation of Plastocyanin mRNA Lacking 5′ Region in the Green Alga <i>Pediastrum boryanum</i> Grown under Copper-Deficient Conditions

Masaki Nakamura, Fuminori Yoshizaki and Yasutomo Sugimura

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 41, issue 1, pages 33-41
Published in print January 2000 | ISSN: 0032-0781
Published online January 2000 | e-ISSN: 1471-9053 | DOI: https://dx.doi.org/10.1093/pcp/41.1.33
Accumulation of Plastocyanin mRNA Lacking 5′ Region in the Green Alga Pediastrum boryanum Grown under Copper-Deficient Conditions

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In the green alga Pediastrum boryanum NIES-301, plastocyanin accumulates under copper-sufficient conditions and cytochrome c6 accumulates under copper-deficient conditions. We cloned the cDNA which encodes pre-apoplastocyanin from P. boryanum cultured under the copper-sufficient condition. The deduced amino acid sequence of the pre-apoplastocyanin protein consists of 151 amino acid residues including a putative bipartite presequence of 53 amino acid residues. Southern blot analysis of P. boryanum genomic DNA indicated that pre-apoplastocyanin is encoded by a single nuclear gene. Northern blot analysis showed that copper-deficient cells accumulated a shorter form of the mRNA of pre-apoplastocyanin, which did not generate pre-apoplastocyanin in the wheat-germ translation system. The difference in size was ascribed to the absence of the 5′ region in the mRNA of pre-apoplastocyanin obtained from the copper-deficient cells, which accounts for the absence of plastocyanin under these conditions. This phenomenon represents a novel regulatory mechanism, although details of the mechanism are not yet known.

Keywords: cDNA sequence; Copper regulation; Green alga; mRNA accumulation; Pediastrum boryanum; Plastocyanin

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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